Description
This product is expressed and purified from Escherichia coli cloned with Thermus aquaticus DNA Polymerase gene. It is free of endonucleases, exonucleases, or bacterial DNA. Taq DNA Polymerase HS is a hot-start Taq enzyme obtained by optimal mixing of specific antibodies with Taq DNA Polymerase. When the reaction is held at 95°C for 30 seconds or longer, the antibodies are completely inactivated, releasing the full activity of Taq polymerase. This ensures high amplification sensitivity and specificity in the PCR systems. Taq DNA Polymerase possesses 5'→3' polymerase activity and 5'→3' exonuclease activity, but lacks 3'→5' exonuclease activity. The PCR product has an A overhang at the 3' end, making it suitable for cloning into T vectors. We have directional protein refolding technology (LeaBioFOLD) and self-developed technology platforms for proteins covering screening and purification, engineering design, fixed-point coupling design, and dosage form development. Protein refolding is a process of recovering protein aggregates in inclusion bodies in the form of misfolded and inactive proteins expressed by prokaryotes such as Escherichia coli back into proteins with correct conformation and bioactivity under appropriate conditions in vitro. It is a key technology for biopharmaceutical companies but a major bottleneck in protein production in prokaryotic expression systems. Leading Biology has been specializing in protein refolding for many years, has a core team with over ten years of experience in this technology and rich experience in its industrialization. Our team is summarizing the experience to form an independent technological system of ours